Temperature Effects on Bacterial Phytochrome

Temperature Effects on Bacterial Phytochrome

Bacteriophytochromes (BphPs) are light-sensing regulatory proteins encoded in photosynthetic and non-photosynthetic bacteria. This protein class incorporate bilin as their chromophore, with majority of them bearing a light- regulated His kinase or His kinase related module in the C-terminal. We studied the His kinase actives in the temperature range of 5°C to 40°C on two BphPs, Agp1 from Agroba...

Temperature Effects on Agrobacterium Phytochrome Agp1

Phytochromes are widely distributed biliprotein photoreceptors with a conserved N-terminal chromophore-binding domain. Most phytochromes bear a light-regulated C-terminal His kinase or His kinase-like region. We investigated the effects of light and temperature on the His kinase activity of the phytochrome Agp1 from Agrobacterium tumefaciens. As in earlier studies, the phosphorylation activity ...

Temperature effects on bacterial movement.

Details are presented for the construction of a simple precision temperature-controlled chamber for investigating bacterial motile behavior. Independent of original incubation temperature, all species of motile bacteria observed showed a five- to sevenfold increase in average translational velocity (micrometers per second) as the environment temperature was incremented over the range from 10 to...

Temperature Tincture: The Effects of Temperature on Bacterial Pigmentation

Objectives/Goals I really enjoyed my project this year. In my project, I tested how temperature affected the pigmentation of four different bacteria. From my research, I knew that the bacterium Serratia marcescens alters its color at different temperatures, and I wanted to explore this. I also wanted to see if this applied to other pigmented bacteria. Methods/Materials My project consisted of q...

Effects of Substrate Solution pH, Incubation Temperature, and Calcination Temperature on Bacterial Synthesis of Nano-Crystalline Hydroxyapatite